Science behind Lassica K014™

Sciences behind

Highly effective in inhibiting polymicrobial growth

Against other pathogenic bacteria and fungus

Besides, Lassica K014™ is found to be equally effective in inhibiting other fungal-bacterial colonisation, including Escherichia coli, Propionibacterium acnes and Candida albicans. This broad-spectrum activity reduces the risks of enhanced pathogenicity of co-infections attributable to polymicrobial interaction of Staphylococcus aureus and Candida albicans in human guts.

Against MRSA

Our studies found that Lassica K014™ is capable of killing 96.1% of Methicillin-resistant Staphylococcus aureus (MRSA) colonies, which is comparable to commercially available antibiotics such as ampicillin, chloramphenicol and vancomycin.

Commensal bacteria and fungi cell counts in the faeces of healthy laboratory rats before and after oral administration of Lassica K014™ within 24 hours

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Under the scanning electron microscopy, it is observed that MRSA cell structures rupture when treated with Lassica K014™ (see figure below).

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Control

Treated with
Lassica K014™

Comparing Anti-MRSA Activities of Lassica K014™ with Antibiotics

Promoting balanced microbiota in gut

Our in-house animal study shows that single dose of Lassica K014™ increases lactic acid bacteria (LAB) cell counts within 24 hours (see diagram below) and reduces the opportunistic pathogens. Studies found that polymicrobial interaction of these opportunistic pathogens in human guts can contribute to enhanced pathogenicity of co-infections. This suggests that the use of Lassica K014™ can have positive impact on gut health.

Inhibitory efficacy % against other pathogens

Escherichia coli

Candida albicans

Propionibacterium acnes

Streptococcus mutans

Stable bioactivity at extreme temperature

Heat Tolerance

While consumers are well aware of the benefits of probiotics for their well-being, the harsh realities of manufacturing processes often compromise the viability and effectiveness of probiotics, limiting their applications.

Lassica K014™ postbiotic can withstand temperatures exceeding 100°C without losing its antimicrobial effectiveness. This exceptional property empowers Lassica K014™ postbiotic to endure a wide array of product manufacturing processes, including pasteurisation (gentle heat treatment below 100°C) and commercial sterilisation (with temperatures surpassing 100°C).

Low pH Tolerance

The journey of probiotics begins with survival in the acidic gastric environment before they can reach the small intestine and provide their health benefits. Unfortunately, most probiotic microorganisms tend to become highly sensitive at pH levels below 3.0.

However, our in-vitro study has unveiled that Lassica K014™ postbiotic's antimicrobial effectiveness remains largely intact even at a pH value of 2.0. This remarkable resilience ensures that it can traverse the gastrointestinal tract without concerns about degradation. Furthermore, the components within Lassica K014™ postbiotic are already inherently functional, eliminating the need for colonisation within the host.

Additional functionalities

Lassica K014™ contains organic antioxidants, which showcases fairly good ABTS radical scavenging activities up to 46.28%. These antioxidants play a crucial role in neutralising harmful free radicals, serving as a protective shield for the skin's surface against oxidative damage.

In addition to its antioxidant prowess, Lassica K014™ also exhibits remarkable anti-inflammatory activities. It can inhibit lipoxygenase (LOX) by as much as 43.7%. The presence of LOX inhibitors in Lassica K014™ effectively hampers LOX from converting arachidonic, linoleic, and other polyunsaturated fatty acids into biologically active metabolites responsible for triggering inflammatory and immune responses. Notably, LOXs also play a pivotal role in the biosynthesis of leukotrienes (LTs), which are closely linked to inflammation-related diseases, including allergic conditions.

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